visualization with processing

visualization with processing
A process by which we could identify a pathogen?

This is a process in which a pathogenic microbes or viruses by using were able to identify DNA identification and visualization techniques? I'm lost on this approach. Thank you!

PCR or chain reaction polymerase. To understand the PCR, first read this article in Wikipedia: http://en.wikipedia.org/wiki/PCR In PCR, generating millions of copies of DNA from tiny amounts of DNA sample and PCR, germs, viruses, plants, worms can, in principle, all organisms are identified. Basically what happened is the DNA of blood or tissue samples obtained even from nasal secretions, swabs nose, hair, etc., so that when the samples containing the virus or microbe, DNA virus or microbe is extracted with the sample. DNA can also be extracted from the pathogen, as they themselves, you can create a bacterial culture from a patient or a virus, you are grown. The DNA is then subjected to PCR with a PCR machine, and we can also compare two primers (primers 18-30 nucleotides are used to strengthen links with the region of DNA that you want. There is a forward and a reverse primer that binds to the 5'-and 3'-ends DNA fragment, respectively). Then, after the PCR reaction, millions of copies of the DNA fragment generated. We then introduce the reactions PCR gel electrophoresis, and you can see the size of the fragment, and we can also follow up on it, and tag them with the GenBank database where the consequences of different organisms are available and we can then identify the organism. There are also some other types of multiplex PCR, nested in real time, etc. Another possibility is to RFLP or restriction polymorphism length. In this method, DNA is extracted and Enzymes restriction digest. Restriction enzymes cut the DNA and they intersect only at certain sequences called recognition sequence (). By example, suppose that X enzyme recognizes the sequence for each AGAA.So AGAA, the enzyme that is X-cut it. After cutting, the fragment sizes DNA produced and can be seen by gel electrophoresis and banding patterns will be generated. Ow by the way they use different primers for different organisms. Some primers are defined as "universal primers" and they are good for gender-specific or a group of viruses or parasites, but you should check the sequence of the PCR product so you know, and the sequence with GenBank, so you can identify. You also find primers that are specific to a single virus or bacterium. So basically everything that is available on the primer. So as you can see numerous articles in journals published on PCR techniques that detect a specific bacterium or virus or a parasite.

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