protein visualization

protein visualization
What is the smallest amount of protein that can be detected by blue dye Coommassie?

In other words, how much protein is essential for the display is. Also, how is it compared to Western Blotting? I can imagine, from the specificity of the antibody.

You can be with Bradford protein quantification at 2 yg / ml, however, it depends on the protein for single Coomassie histidine and arginine patch of aromatic amino acids, so the correct answer It depends on the amino acid composition of your protein.however this staining is not specific, it will stain all proteins. In contrast, Western blot is specific for a protein and also more sensitive, but again it depends on the methods, with 2-antibodies, but the most common, which is more sensitive than Coomassie definitevely

Two-Level Approach to Efficient Visualization of Protein Dyn